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Characterization of miRNomes in Acute and Chronic Myeloid Leukemia Cell Lines

Authors :
Xiong, Qian
Yang, Yadong
Wang, Hai
Li, Jie
Wang, Shaobin
Li, Yanming
Yang, Yaran
Cai, Kan
Ruan, Xiuyan
Yan, Jiangwei
Hu, Songnian
Fang, Xiangdong
Source :
Genomics, Proteomics and Bioinformatics; April 2014, Vol. 12 Issue: 2 p79-91, 13p
Publication Year :
2014

Abstract

Myeloid leukemias are highly diverse diseases and have been shown to be associated with microRNA (miRNA) expression aberrations. The present study involved an in-depth miRNome analysis of two human acute myeloid leukemia (AML) cell lines, HL-60 and THP-1, and one human chronic myeloid leukemia (CML) cell line, K562, via massively parallel signature sequencing. mRNA expression profiles of these cell lines that were established previously in our lab facilitated an integrative analysis of miRNA and mRNA expression patterns. miRNA expression profiling followed by differential expression analysis and target prediction suggested numerous miRNA signatures in AML and CML cell lines. Some miRNAs may act as either tumor suppressors or oncomiRs in AML and CML by targeting key genes in AML and CML pathways. Expression patterns of cell type-specific miRNAs could partially reflect the characteristics of K562, HL-60 and THP-1 cell lines, such as actin filament-based processes, responsiveness to stimulus and phagocytic activity. miRNAs may also regulate myeloid differentiation, since they usually suppress differentiation regulators. Our study provides a resource to further investigate the employment of miRNAs in human leukemia subtyping, leukemogenesis and myeloid development. In addition, the distinctive miRNA signatures may be potential candidates for the clinical diagnosis, prognosis and treatment of myeloid leukemias.

Details

Language :
English
ISSN :
16720229
Volume :
12
Issue :
2
Database :
Supplemental Index
Journal :
Genomics, Proteomics and Bioinformatics
Publication Type :
Periodical
Accession number :
ejs66328028
Full Text :
https://doi.org/10.1016/j.gpb.2014.02.001