Sources and Prevalence of Cyclospora cayetanensisin Southeastern U.S. Growing Environments

•Ag water, wastewater, and portable toilets were tested for C. cayetanensis.•C. cayetanensisqPCR detections from wastewater were confirmed by sequencing.•qPCR detections from water couldn’t be confirmed, suggesting false detection.•A single method may not be able to discern C. cayetanensisfrom other Eimeriidae.