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Rational Design of Triazinone Derivatives with Low Bee Toxicity Based on the Binding Mechanism of Neonicotinoids to Apis mellifera
- Source :
- Journal of Agricultural and Food Chemistry; June 2024, Vol. 72 Issue: 23 p12956-12966, 11p
- Publication Year :
- 2024
-
Abstract
- Bees, one of the most vital pollinators in the ecosystem and agriculture, are currently threatened by neonicotinoids. To explore the molecular mechanisms of neonicotinoid toxicity to bees, the different binding modes of imidacloprid, thiacloprid, and flupyradifurone with nicotinic acetylcholine receptor (nAChR) α1β1 and cytochrome P450 9Q3 (CYP9Q3) were studied using homology modeling and molecular dynamics simulations. These mechanisms provided a basis for the design of compounds with a potential low bee toxicity. Consequently, we designed and synthesized a series of triazinone derivatives and assessed their bioassays. Among them, compound 5anot only displayed substantially insecticidal activities against Aphis glycines(LC50= 4.40 mg/L) and Myzus persicae(LC50= 6.44 mg/L) but also had low toxicity to Apis mellifera. Two-electrode voltage clamp recordings further confirmed that compound 5ainteracted with the M. persicaenAChR α1 subunit but not with the A. melliferanAChR α1 subunit. This work provides a paradigm for applying molecular toxic mechanisms to the design of compounds with low bee toxicity, thereby aiding the future rational design of eco-friendly nicotinic insecticides.
Details
- Language :
- English
- ISSN :
- 00218561 and 15205118
- Volume :
- 72
- Issue :
- 23
- Database :
- Supplemental Index
- Journal :
- Journal of Agricultural and Food Chemistry
- Publication Type :
- Periodical
- Accession number :
- ejs66517787
- Full Text :
- https://doi.org/10.1021/acs.jafc.4c00679