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Investigating the Role of KLF6in the Growth of Bovine Preadipocytes: Using Transcriptomic Analyses to Understand Beef Quality

Investigating the Role of KLF6in the Growth of Bovine Preadipocytes: Using Transcriptomic Analyses to Understand Beef Quality

Authors :
Abbas Raza, Sayed Haidar
Zhong, Ruimin
Wei, Xiaoqun
Zhao, Gang
Zan, Linsen
Pant, Sameer D.
Schreurs, Nicola M.
Lei, Hongtao
Source :
Journal of Agricultural and Food Chemistry; May 2024, Vol. 72 Issue: 17 p9656-9668, 13p
Publication Year :
2024

Abstract

Intramuscular fat is a crucial determinant of carcass quality traits like tenderness and taste, which in turn is influenced by the proliferation of intramuscular preadipocytes. This study aimed to investigate the Kru¨ppel-like factor 6 (KLF6)-mediated proliferation of bovine preadipocytes and identify underlying molecular mechanisms. Down-regulation of KLF6 by siKLF6 resulted in a significant (p< 0.01) suppression of cell cycle-related genes including CDK1, MCM6, ZNF4, PCNA, CDK2, CCNB1, and CDK6. Conversely, the expression level of p27 was significantly (p< 0.01) increased. Moreover, EdU (5-ethynyl-20-deoxyuridine) staining revealed a significant decrease in EdU-labeled cells due to KLF6 down-regulation. Collectively, these findings indicate that KLF6 down-regulation inhibits adipocyte proliferation. Furthermore, RNA sequencing of preadipocytes transfected with siKLF6 and NC, followed by differential gene expression analysis, identified 100 up-regulated and 70 down-regulated genes. Additionally, the differentially expressed genes also significantly influenced various Gene Ontology (GO) terms related to cell cycle, nuclear chromosomes, and catalytic activity on DNA. Furthermore, the top 20 pathways enriched in these DEGs included cell cycle, DNA replication, cellular senescence, and homologous recombination. These GO terms and KEGG pathways play key roles in bovine preadipocyte proliferation. In conclusion, the results of this study suggest that KLF6 positively regulates the proliferation of bovine preadipocytes.

Details

Language :
English
ISSN :
00218561 and 15205118
Volume :
72
Issue :
17
Database :
Supplemental Index
Journal :
Journal of Agricultural and Food Chemistry
Publication Type :
Periodical
Accession number :
ejs67017566
Full Text :
https://doi.org/10.1021/acs.jafc.4c01115