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Mechanism of Phloretin Accumulation in Malus hupehensisGrown at High Altitudes: Evidence from Quantitative 4D Proteomics

Authors :
Li, Qing
Wang, Shanshan
Wang, Junzhi
Chen, Lijun
Liu, Wenrui
Li, Ziyan
Xu, Jingyuan
Deng, Zhangshuang
Zhou, Yiqing
Source :
Journal of Agricultural and Food Chemistry; September 2024, Vol. 72 Issue: 35 p19526-19536, 11p
Publication Year :
2024

Abstract

Phloretin is a natural dihydrochalcone (DHC) that exhibits various pharmacological and therapeutic activities. Malus hupehensisRehd. (M. hupehensis) is widely planted in the middle of China and its leaves contain an extremely high content of phloridzin, a glycosylated derivative of phloretin. In the present study, we observed a significant increase in phloretin content in the leaves of M. hupehensisplanted at high altitudes. To investigate the mechanisms of phloretin accumulation, we explored changes in the proteome profiles of M. hupehensisplants grown at various altitudes. The results showed that at high altitudes, photosynthesis- and DHC biosynthesis-related proteins were downregulated and upregulated, respectively, leading to reduced chlorophyll content and DHC accumulation in the leaves. Moreover, we identified a novel phloridzin-catalyzing glucosidase whose expression level was significantly increased in high-altitude-cultivated plants. This work provided a better understanding of the mechanism of phloretin accumulation and effective and economic strategies for phloretin production.

Details

Language :
English
ISSN :
00218561 and 15205118
Volume :
72
Issue :
35
Database :
Supplemental Index
Journal :
Journal of Agricultural and Food Chemistry
Publication Type :
Periodical
Accession number :
ejs67205634
Full Text :
https://doi.org/10.1021/acs.jafc.4c04487