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Identification and Characterization of a Novel SH3-Domain Binding Protein, Sab, Which Preferentially Associates with Bruton's Tyrosine Kinase (Btk)
- Source :
- Biochemical and Biophysical Research Communications; April 1998, Vol. 245 Issue: 2 p337-343, 7p
- Publication Year :
- 1998
-
Abstract
- Protein interaction cloning method was used to identify a novel molecule, Sab, which binds to the SH3 domain of Bruton's tyrosine kinase (Btk), the deficient cytoplasmic tyrosine kinase in human X-linked agammaglobulinemia and murine X-linked immunodeficiency. Immunoprecipitation using the anti-Sab antibody identified the protein product of the gene as a 70 kDa molecule. While Sab does not have a proline-rich sequence, it was shown to bind to Btk through the commonly conserved structure among SH3 domains. Remarkably, Sab exhibited a high preference for binding to Btk rather than to other cytoplasmic tyrosine kinases, which suggests a unique role of Sab in the Btk signal transduction pathway.
Details
- Language :
- English
- ISSN :
- 0006291X and 10902104
- Volume :
- 245
- Issue :
- 2
- Database :
- Supplemental Index
- Journal :
- Biochemical and Biophysical Research Communications
- Publication Type :
- Periodical
- Accession number :
- ejs674158
- Full Text :
- https://doi.org/10.1006/bbrc.1998.8420