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An Epithelial-dependent Contracting Factor Induced by Calcium Influx in Guinea-pig Trachea
- Source :
- Pulmonary Pharmacology; March 1993, Vol. 6 Issue: 1 p69-76, 8p
- Publication Year :
- 1993
-
Abstract
- Summary: The effects of epithelium removal were studied on the contraction induced by Ca<SUP>2+</SUP> in K<SUP>+</SUP>-depolarizing solution, and by the calcium ionophore A 23187 in guinea-pig isolated tracheal strips. Epithelium removal reduced the maximal response to Ca<SUP>2+</SUP> in K<SUP>+</SUP>-depolarizing solution and caused a significant shift to the right of the Ca<SUP>2+</SUP> concentration-response curves. The contraction induced by the calcium ionophore A 23187 (10<SUP>-6</SUP> M) was also markedly reduced by epithelium removal. These results suggest the occurrence of an epithelium-derived contracting factor. The effects of hexamethonium, atropine, spantide and thiorphan showed that acetylcholine and neurokinins play a minor role in the Ca<SUP>2+</SUP>-induced contraction. The epithelium-dependent potentiation of the calcium- and of the A 23187-induced contractions was inhibited by an antibody selective for rat calcitonin gene-related peptide (rCGRPα). Therefore, CGRP-like immunoreactive material may be part of the epithelium-dependent contracting factor of guinea-pig trachea. Comparison of concentration-response curves for rCGRPα in epithelium-free and in intact guinea-pig tracheal strips suggests that an epithelium-dependent contracting factor may be mobilized by CGRP. Copyright 1993, 1999 Academic Press
Details
- Language :
- English
- ISSN :
- 09520600 and 15229637
- Volume :
- 6
- Issue :
- 1
- Database :
- Supplemental Index
- Journal :
- Pulmonary Pharmacology
- Publication Type :
- Periodical
- Accession number :
- ejs715505
- Full Text :
- https://doi.org/10.1006/pulp.1993.1010