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An Epithelial-dependent Contracting Factor Induced by Calcium Influx in Guinea-pig Trachea

Authors :
Bertrand, C.
Silva, A. Da
Landry, Y.
Theodorsson, E.
Tschirhart, E.
Source :
Pulmonary Pharmacology; March 1993, Vol. 6 Issue: 1 p69-76, 8p
Publication Year :
1993

Abstract

Summary: The effects of epithelium removal were studied on the contraction induced by Ca<SUP>2+</SUP> in K<SUP>+</SUP>-depolarizing solution, and by the calcium ionophore A 23187 in guinea-pig isolated tracheal strips. Epithelium removal reduced the maximal response to Ca<SUP>2+</SUP> in K<SUP>+</SUP>-depolarizing solution and caused a significant shift to the right of the Ca<SUP>2+</SUP> concentration-response curves. The contraction induced by the calcium ionophore A 23187 (10<SUP>-6</SUP> M) was also markedly reduced by epithelium removal. These results suggest the occurrence of an epithelium-derived contracting factor. The effects of hexamethonium, atropine, spantide and thiorphan showed that acetylcholine and neurokinins play a minor role in the Ca<SUP>2+</SUP>-induced contraction. The epithelium-dependent potentiation of the calcium- and of the A 23187-induced contractions was inhibited by an antibody selective for rat calcitonin gene-related peptide (rCGRPα). Therefore, CGRP-like immunoreactive material may be part of the epithelium-dependent contracting factor of guinea-pig trachea. Comparison of concentration-response curves for rCGRPα in epithelium-free and in intact guinea-pig tracheal strips suggests that an epithelium-dependent contracting factor may be mobilized by CGRP. Copyright 1993, 1999 Academic Press

Details

Language :
English
ISSN :
09520600 and 15229637
Volume :
6
Issue :
1
Database :
Supplemental Index
Journal :
Pulmonary Pharmacology
Publication Type :
Periodical
Accession number :
ejs715505
Full Text :
https://doi.org/10.1006/pulp.1993.1010