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A Plant-specific Cyclin-dependent Kinase Is Involved in the Control of G2/M Progression in Plants*
- Source :
- Journal of Biological Chemistry; September 2001, Vol. 276 Issue: 39 p36354-36360, 7p
- Publication Year :
- 2001
-
Abstract
- Cyclin-dependent kinases (CDKs) control the key transitions in the eukaryotic cell cycle. All the CDKs known to control G2/M progression in yeast and animals are distinguished by the characteristic PSTAIRE motif in their cyclin-binding domain and are closely related. Higher plants contain in addition a number of more divergent non-PSTAIRE CDKs with still obscure functions. We show that a plant-specific type of non-PSTAIRE CDKs is involved in the control of the G2/M progression. In synchronized tobacco BY-2 cells, the corresponding protein, accumulated in a cell cycle-regulated fashion, peaking at the G2/M transition. The associated histone H1 kinase activity reached a maximum in mitosis and required a yet unidentified subunit to be fully active. Down-regulation of the associated kinase activity in transgenic tobacco plants using a dominant-negative mutation delayed G2/M transition. These results provide the first evidence that non-PSTAIRE CDKs are involved in the control of the G2/M progression in plants.
Details
- Language :
- English
- ISSN :
- 00219258 and 1083351X
- Volume :
- 276
- Issue :
- 39
- Database :
- Supplemental Index
- Journal :
- Journal of Biological Chemistry
- Publication Type :
- Periodical
- Accession number :
- ejs7175412
- Full Text :
- https://doi.org/10.1074/jbc.M011060200