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Forced Expression of YL-1 Protein Suppresses the Anchorage-Independent Growth of Kirsten Sarcoma Virus-Transformed NIH3T3 Cells
- Source :
- Experimental Cell Research; September 1995, Vol. 220 Issue: 1 p11-17, 7p
- Publication Year :
- 1995
-
Abstract
- The YL-1gene, encoding a novel nuclear protein with transcription factor-like features, has been isolated from the human chromosome 1q21, one of the regions supposedly carrying a transformation suppressor gene(s) for Kirsten sarcoma virus-transformed NIH3T3 (DT) cells. To test the suppressive activity of the YL-1gene product, we forced the expression of human YL-1cDNA in DT cells. The anchorage-independent growth (colony-forming ability in soft agar medium) was markedly suppressed in cells highly expressing the exogenous human YL-1 protein. Moreover, the soft agar clones, which were rarely originated from these cells, expressed reduced levels of exogenous YL-1or none, with or without the loss/rearrangement of the introduced cDNA. In control experiments, cells carrying an introduced vector alone or an antisense-strand expression plasmid grew in soft agar as efficiently as parental DT cells. In contrast to the suppression of anchorage-independent growth, the forced expression of YL-1did not affect the transformed phenotypes in adherent culture and tumorigenicity in nude mice. These findings not only indicated that the YL-1 protein functions as a transformation suppressor, but also suggest that it may be important for elucidating anchorage independence under separate genetic control from other transformed phenotypes.
Details
- Language :
- English
- ISSN :
- 00144827 and 10902422
- Volume :
- 220
- Issue :
- 1
- Database :
- Supplemental Index
- Journal :
- Experimental Cell Research
- Publication Type :
- Periodical
- Accession number :
- ejs719538
- Full Text :
- https://doi.org/10.1006/excr.1995.1286