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X-linked heterochromatin distribution in the holocentric chromosomes of the green apple aphid Aphis pomi
- Source :
- Genetica; May 2005, Vol. 124 Issue: 1 p93-98, 6p
- Publication Year :
- 2005
-
Abstract
- Abstract Chromatin organization in the holocentric chromosomes of the green apple aphid Aphis pomi has been investigated at a cytological level after C-banding, NOR, Giemsa, fluorochrome staining and fluorescent in situ hybridization (FISH). C-banding technique showed that heterochromatic bands are exclusively located on X chromosomes. This data represents a peculiar feature that clearly contradicts the equilocal distribution of heterochromatin typical of monocentric chromosomes. Moreover, silver staining and FISH carried out with a 28S rDNA probe localized rDNA genes on one telomere of each X chromosome; CMA3 staining reveals that these silver positive telomeres are the only GC-rich regions among A. pomi heterochromatin, whereas all other C-positive bands are DAPI positive thus containing AT-rich DNA.
Details
- Language :
- English
- ISSN :
- 00166707 and 15736857
- Volume :
- 124
- Issue :
- 1
- Database :
- Supplemental Index
- Journal :
- Genetica
- Publication Type :
- Periodical
- Accession number :
- ejs7215575
- Full Text :
- https://doi.org/10.1007/s10709-004-8154-y