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Efficient In Vivo Xenogeneic Retroviral Vector-Mediated Gene Transduction into Human Hepatocytes
- Source :
- Human Gene Therapy; October 2005, Vol. 16 Issue: 10 p1168-1174, 7p
- Publication Year :
- 2005
-
Abstract
- We developed a method for efficient retroviral vector-mediated gene transfer into human hepatocytes, using a human hepatocyte-bearing mouse model. Normal human hepatocytes were transplanted into the livers of immunodeficient and liver-damaged mice. Donor hepatocytes multiplied and replaced the host hepatocytes, which yielded human hepatocyte-bearing mice (human hepatocyte-chimeric mice). As control cells, rat hepatocytes were similarly transplanted. The replacement level reached 86% at 8 weeks and 100% at 5 weeks posttransplantation of human and rat hepatocytes, respectively. Human and rat hepatocytes in the host liver showed a high bromodeoxyuridine-labeling index during the first 2 weeks posttransplantation. Human- and rat-chimeric mice were injected 7 and 10 days posttransplantation, respectively, with retroviral vectors carrying the β-galactosidase gene and were thereafter injected daily for 20 and 10 days, respectively. The level of β-galactosidase-positive hepatocytes in the human- and rat-chimeric mice reached 7.1 ± 1.8% at 8 weeks and 5.3 ± 0.9% at 5 weeks after transplantation, respectively. The human hepatocyte-chimeric mouse will be useful for testing the ability of vectors to transduce human cells.
Details
- Language :
- English
- ISSN :
- 10430342 and 15577422
- Volume :
- 16
- Issue :
- 10
- Database :
- Supplemental Index
- Journal :
- Human Gene Therapy
- Publication Type :
- Periodical
- Accession number :
- ejs7783135
- Full Text :
- https://doi.org/10.1089/hum.2005.16.1168