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Electrochemical Detection of Abasic Site‐Containing DNA

Authors :
Dolinnaya, Nina G.
Jan, Mohammad Rasul
Kawde, Abdel‐Nasser
Oretskaya, Tatiana S.
Tashlitsky, Vadim N.
Wang, Joseph
Source :
Electroanalysis; February 2006, Vol. 18 Issue: 4 p399-404, 6p
Publication Year :
2006

Abstract

A simple and rapid approach for detecting apurinic (AP) sites in DNA, based on direct stripping chronopotentiometric measurements of the adenine and guanine nucleobases at a graphite electrode is described. Tetrahydrofuranyl residues, lacking a nucleobase moiety, were utilized for designing the AP sites and were incorporated in 19‐mer oligonucleotides. The change of adenine‐to‐guanine response ratio (A/G) in one‐, two‐ or three‐substituted adenosine residues for stable analogs of AP sites was exploited for electrochemical measurements of the adenine loss. The resulting A/G response ratio decreases linearly upon increasing the number of AP sites in the oligonucleotides; the values of A/G electrochemical signals were slightly enhanced when compared to the actual purine content. HPLC analysis of the released nucleobases confirmed that the sulfuric acid‐induced oligonucleotide cleavage provides complete apurination and dissolution of the released nucleobases in aqueous solution. Additional experiments with mixtures of free nucleobases and purine nucleosides reveal that the larger A/G ratio observed in the electrochemical analysis of AP‐site‐containing oligomers is attributed to the influence of the acid and/or thermal decomposition products (particularly the sugar fragments). This study represents the first step in developing a simple and direct electrochemical assay of AP sites in single‐stranded DNA.

Details

Language :
English
ISSN :
10400397 and 15214109
Volume :
18
Issue :
4
Database :
Supplemental Index
Journal :
Electroanalysis
Publication Type :
Periodical
Accession number :
ejs8527849
Full Text :
https://doi.org/10.1002/elan.200503428