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Enhancing Efficacy of HIV Gag DNA Vaccine by Local Delivery of GM-CSF in Murine and Macaque Models
- Source :
- Journal of Interferon & Cytokine Research; June 2006, Vol. 26 Issue: 6 p380-389, 10p
- Publication Year :
- 2006
-
Abstract
- Controlled release of granulocyte-macrophage colony-stimulating factor (GM-CSF) protein by albumin-heparin microparticles administered via intramuscular vaccination in conjunction with HIV DNA vaccines stimulated HIV Gag-specific immune responses. In the murine model, Gag-specific cytotoxic T lymphocyte (CTL) and T helper (Th) responses were significantly enhanced by administration of murine GM-CSF microparticles. This effect was comparable to a GM-CSF encoded plasmid. In three of four rhesus monkeys, enhancement of Gag-specific antibody (Ab), Th, and CTL responses was observed 1 month after the first immunization with coadministration of human GM-CSF microparticles and HIV Gag plasmid. The second, third, and fourth booster immunizations, however, did not increase the Gag-specific immune responses. Subsequent application of Gag protein in complete Freund's adjuvant (CFA) significantly enhanced Ab and Th, but not CTL. However, Gag-specific CTL response was triggered by cytokine and Gag p55-encapsulated microparticles in all animals. The strategy of priming immune responses by coadministration of cytokine microparticles and DNA vaccines, followed by boosting with cytokine and antigen protein-encapsulated microparticles, may prove effective in improving an HIV DNA vaccine design.
Details
- Language :
- English
- ISSN :
- 10799907 and 15577465
- Volume :
- 26
- Issue :
- 6
- Database :
- Supplemental Index
- Journal :
- Journal of Interferon & Cytokine Research
- Publication Type :
- Periodical
- Accession number :
- ejs9075543
- Full Text :
- https://doi.org/10.1089/jir.2006.26.380