The influence of ozone on retention of sealing material: a clinical study

Introduction: The aim of the present study is to clinically analyse the influence of pre- treatment of enamel with ozone prior to fissure sealing with flowable composite material, on retention of the seal. Retention of the nanohybrid flowable composite material as a material for fissure sealing was also analysed through the clinical period of 24 months. Materials and methods: GroupAconsisted of 79 healthy permanent molars who were treated with ozone from KaVo Healozone device before the application of Voco Grandio Flow flowable composite. Group B consisted of 75 healthy permanent molars who were also sealed with Voco Grandio Flow but without the pre-treatment with ozone. Testing of the material’ s retention was conducted after 24 months of clinical use. Following values were assessed: 0- complete retention, 1-loss of 1/3, 2-loss of 2/3, 3- complete loss of seal, 4-caries. Chi square test was used for statistical analysis.Results: After 24 months of clinical use, complete retention was found on 82.3% of the teeth in group A, and 84% in group B. Caries was found in 1.3% of the cases in group B only. No statistically significant differences between the groups (v2 = 1.976 ; P = 0.577) were found. Conclusion: Considering the fact that ozone had no negative effect on retention of the sealing material after 24 months, we concluded that it can be used as pre-treatment of enamel before fissure sealing. Sealing the fissures with nanohybrid flowable composite showed a high degree of retention and consequently excellent prevention of occlusal caries.