Mikroekstrakcija zapakiranim sorbensom derivata andrografolida u ljudskoj plazmi

Andrographolides are diterpenoids found in the plant of Andrographis paniculata (Burm.f.) Nees (Acanthaceae) and widely used in traditional medicine, exhibiting anti-inflammatory property. In this research, a novel micro-extraction by packed sorbent (MEPS) procedure for sample preparation was examined with the aim of extraction of pharmacological active andrographolide, neoandrographolide and 14- deoxyandrographolide from plasma samples. The analysis of samples was performed using developed and validated high-performance liquid chromatography with diode array detection (HPLC/DAD) method. The validation results showed acceptable linearity of the method (R2>0.99) for all analytes within the calibration range. Acceptable accuracy and precision of the method were indicated by recovery of 91–100% and Relative Standard Deviation (RSD) values below 7.6%.The extraction recovery was found to be between 91.04 and 100.34% with RSD values not higher than 11.5%. The limit of quantification was found to be between 62 and 322 ng/mL while the limit of detection was found between 18 and 97 ng/mL. Short-term (1 - 3 days at room temperature) and long-term stability (3 days at -80 °C) were also tested and showed no significant difference (RSDs were lower than 14.8%). The development of this fast and easy MEPS pretreatment procedure granted agreeable validation results and it represents a bioanalytical improvement (cheaper, greener and less time-consuming) over classical solid-phase extraction methods.