Razvoj integriranog bioprocesa za proizvodnju etanola iz šećerne repe

Ethanol production (as a biofuel) from renewable raw materials is still more expensive than gasoline production from fossil oil. Therefore, integration of different technological stages into one single stage could result in a more cost-effective and energy saving bioprocess. In this research, intermediates of sugar beet processing were studied as a substrate for ethanol production in order to reduce ethanol price as a biofuel. For ethanol production from the raw sugar beet juice in the stirred tank bioreactor batch and fed batch cultivation techniques were used. In horizontal rotating tubular bioreactor (HRTB) batch ethanol production from raw sugar beet cossettes was studied by different initial inoculum quantity (9.1 - 23.1 v/m). In both cases, Saccharomyces cerevisiae was used as a production microorganism. During batch ethanol production from the raw sugar beet juice, bioprocess efficiency was 59.48 % and in the fed batch process 72.49 %, respectively. At the same time, ethanol production in the HRTB from the raw sugar beet cossettes with inoculum of 16.7 % v/m (raw sugar beet cossettes) resulted in the highest bioprocess efficiency of 79.92 %. On the basis of experimental data it is clear that both intermediates of sugar beet processing can be successfully used for ethanol production.