Primjena pektina iz kore mandarine (C. reticulata) u biogenoj sintezi selenovih nanočestica

Mandarin peel has been recognized as the valuable source of functional ingredients such as polyphenols (hesperidin, tangeretin and nobiletin) and dietary fibres (pectin). In this study, we optimized the procedure of extraction and purification of pectin from mandarin peel extract for using raw pectin fraction (RP) and purified pectin fraction (PP) in biogenic synthesis of selenium nanoparticles (bnSeNPs). Obtained SeNPs were compared to chemically synthesised particles (cnSeNPs) in terms of size distribution, zeta potential, stability and functional properties (antiradical and reductive ability). The bnSeNPs stabilized with 0.7 % RP and PP showed particle size distribution comparable to cnSeNPs with average diameter of 230 nm and 290 nm, respectively. Surface charge of bnSeNPs showed more negative values than for cnSeNPs (-35 mV in relation to -10 mV) indicating better stability of obtained colloidal system. Also, in comparison to cnSe, bnSe coated with 0.7 % RP and PP showed significantly higher antioxidant activity (24.5 and 46.3 gTE molSe-1, respectively) in comparison to cnSeNPs (1.91 gTE molSe-1) that were probably associated to polyphenols remaining in pectin fractions. Obtained results revealed the potential of mandarin peel as a source of natural coating materials for development of green synthesis processes of SeNPs achieving improved functional properties.