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Rb binding protein Che-1 interacts with Tau cerebellar granule neurons. Modulation during neuronal apoptosis

Authors :
Barbato C
Corbi N
Canu N
Fanciulli M
Serafino A
Ciotti M
Libri V
Bruno T
Amadoro G
De Angelis R
Calissano P
Passananti C.
Source :
Molecular and cellular neurosciences, 24 (2002): 1038–1050., info:cnr-pdr/source/autori:Barbato C, Corbi N, Canu N, Fanciulli M, Serafino A, Ciotti M, Libri V, Bruno T, Amadoro G, De Angelis R, Calissano P, Passananti C./titolo:Rb binding protein Che-1 interacts with Tau cerebellar granule neurons. Modulation during neuronal apoptosis./doi:/rivista:Molecular and cellular neurosciences (Print)/anno:2002/pagina_da:1038/pagina_a:1050/intervallo_pagine:1038–1050/volume:24
Publication Year :
2002
Publisher :
Academic Press, San Diego , Stati Uniti d'America, 2002.

Abstract

Che-1 is a recently identified human Rb binding protein that inhibits the Rb growth-suppressing function and regulates cell proliferation. Che-1 contacts the Rb and competes with HDAC1 for Rb-binding site, removing HDAC1 from the Rb/E2F cell cycle-regulated promoters. We have investigated the expression of Che-1 in neuronal cells and we showed that Che-1 directly interacts with Tau. Tau is a microtubule-associated protein involved in the assembly and stabilization of neuronal microtubules network that plays a crucial role modulating neuronal morphogenesis, axonal shape, and transport. In rat cerebellar granule neurons (CGNs) Che-1 partially colocalizes with Tau in the cytoplasm. Che-1 binds the amino-terminal region of Tau protein, which is not involved in microtubule interactions. Tau and Che-1 endogenous proteins coimmunoprecipitate from CGNs cellular lysates. In addition, Che-1/Tau interaction was demonstrated both in overexpressing COS-7 cells and CGNs by FRET analysis. Finally, we observed that Tau/Che-1 interaction is modulated during neuronal apoptosis.

Details

Database :
OpenAIRE
Journal :
Molecular and cellular neurosciences, 24 (2002): 1038–1050., info:cnr-pdr/source/autori:Barbato C, Corbi N, Canu N, Fanciulli M, Serafino A, Ciotti M, Libri V, Bruno T, Amadoro G, De Angelis R, Calissano P, Passananti C./titolo:Rb binding protein Che-1 interacts with Tau cerebellar granule neurons. Modulation during neuronal apoptosis./doi:/rivista:Molecular and cellular neurosciences (Print)/anno:2002/pagina_da:1038/pagina_a:1050/intervallo_pagine:1038–1050/volume:24
Accession number :
edsair.cnr...........eac0c5987e8a82582112392920a64e8f