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Evolutionary relationship between Old World West Nile virus strains Evidence for viral gene flow between africa, the middle east, and europe
- Source :
- Virology. (2):381-388
- Publisher :
- Elsevier Inc.
-
Abstract
- Little is known about the genetic relationships between European and other Old-World strains of West Nile virus (WNV) and persistence of WNV North of Mediterranean. We characterized the complete genomes of three WNV strains from France (horse-2000), Tunisia (human-1997) and Kenya (mosquito-1998), and the envelope, NS3 and NS5 genes of the Koutango virus. Phylogenetic analyses including all available full-length sequences showed that: (1) Koutango virus is a distant variant of WNV; (2) the three characterized strains belong to lineage 1, clade 1a; (3) the Tunisian strain roots the lineage of viruses introduced in North America. We established that currently available partial envelope sequences do not generate reliable phylogenies. Accordingly, establishing a large WNV sequence database is pivotal for the understanding of spatial and temporal epidemiology of this virus. For rapid completion of that purpose, colinearized E-NS3-NS5 gene sequences were shown to constitute a valuable surrogate for complete sequences.
- Subjects :
- viruses
virus diseases
Subjects
Details
- Language :
- English
- ISSN :
- 00426822
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Virology
- Accession number :
- edsair.core.ac.uk....b2b224aef491cb06f5862075a66c47b6
- Full Text :
- https://doi.org/10.1016/S0042-6822(03)00536-1