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NEMO-binding domain peptide inhibits proliferation of human melanoma cells

Authors :
IANARO, ANGELA
IALENTI, ARMANDO
M. Tersigni
Belardo G
Di Martino S
Napolitano M
Palmieri G
Sini M
DE MAIO, ANNA
Ombra M
Gentilcore G
Capone M
Ascierto M
Satriano RA
Farina B
FARAONE MENNELLA, MARIA ROSARIA
Ascierto PA
Ianaro, A
Tersigni, M
Belardo, G
Martino, Sd
Napolitano, M
Palmieri, G
Sini, M.
DE MAIO, Anna
Ombra, M
Gentilcore, G
Capone, M
Ascierto, M
Satriano, Ra
Farina, B
FARAONE MENNELLA, MARIA ROSARIA
Ascierto, Pa
Ialenti, A.
Ianaro, Angela
M., Tersigni
Di Martino, S
Sini, M
Ialenti, Armando
Source :
Cancer letters, 274 (2009): 331–336., info:cnr-pdr/source/autori:Ianaro A, Tersigni M, Belardo G, Di Martino S, Napolitano M, Palmieri G, Sini M, Maio AD, Ombra M, Gentilcore G, Capone M, Ascierto M, Satriano RA, Farina B, Faraone-Mennella M, Ascierto PA, Ialenti A./titolo:NEMO-binding domain peptide inhibits proliferation of human melanoma cells./doi:/rivista:Cancer letters (Print)/anno:2009/pagina_da:331/pagina_a:336/intervallo_pagine:331–336/volume:274
Publication Year :
2009

Abstract

Melanoma is the most aggressive form of skin cancer, it originates from melanocytes and its incidence has increased in the last decade. Recent advances in the understanding of the underlying biology of the progression of melanoma have identified key signalling pathways that are important in promoting melanoma tumourigenesis, thus providing dynamic targets for therapy. One such important target identified in melanoma tumour progression is the Nuclear Factor-kB (NF-kB) pathway. In vitro studies have shown that NF-kB binding is constitutively elevated in human melanoma cultures compared to normal elanocytes. It has been found that a short cell-permeable peptide spanning the IKK-beta NBD, named NBD peptide, disrupted the association of NEMO with IKKs in vitro and blocked TNFalpha-induced NF-kB activation in vivo. In the present study we investigated the effect of the NBD peptide on NF-kB activity and survival of A375 human melanoma cells. We found that NBD peptide is able to inhibit the proliferation of A375 cells, which present constitutively elevated NF-kB levels. Inhibition of cell proliferation by NBD peptide was associated with direct inhibition of constitutive NF-kB DNA-binding activity and induction of apoptosis by activation of caspase-3 as confirmed by the cleavage and consequently inactivation of poly (ADP ribose) polymerase (PARP-1) known as the best marker of this process.

Subjects

Subjects :
NEMO
melanoma

Details

Language :
English
Database :
OpenAIRE
Journal :
Cancer letters, 274 (2009): 331–336., info:cnr-pdr/source/autori:Ianaro A, Tersigni M, Belardo G, Di Martino S, Napolitano M, Palmieri G, Sini M, Maio AD, Ombra M, Gentilcore G, Capone M, Ascierto M, Satriano RA, Farina B, Faraone-Mennella M, Ascierto PA, Ialenti A./titolo:NEMO-binding domain peptide inhibits proliferation of human melanoma cells./doi:/rivista:Cancer letters (Print)/anno:2009/pagina_da:331/pagina_a:336/intervallo_pagine:331–336/volume:274
Accession number :
edsair.dedup.wf.001..215f73148c7479f625b53501f7781dd0