THE EFFECTS OF LONG-TERM DIABETES MELLITUS ON ADULT NEUROGENESIS IN SUBVENTRICULAR ZONE OF THE RAT BRAIN

Objectives: Diabetes mellitus has been associated with increased risk of cognitive decline, dementia, depression and anxiety. The aims of this study were to determine the influence of aging and long-term type 1 diabetes mellitus (DM1) on the rate of adult neurogenesis in subventricular zone of rats, as well as to determine the possible role of DSTYK protein in the adult neurogenesis in subventricular zone of rats as a possible disease mechanism. Material and Methods: Thirty-five male Sprague-Dawley rats randomly divided into two groups: control or diabetic. DM1 was induced by intraperitoneal injection of streptozotocin (55 mg/kg). DM1 was confirmed by measuring of plasma glucose 4 days after induction. Rats were sacrificed 2 weeks, 6 months and 1 year after induction of the DM1. Tissues of brain were processed for the immunohistochemistry for doublecortin (DCX) alone, or in combination with DSTYK. Results: For the thick part of the subependymal layer of the SVZ, the percentage of DCX area in the sections from a control group significantly decreased 6 months in comparison to the 2 weeks after the beginning of the experiment (P
Ciljevi: Šećerna bolest povezana je s povećanim rizikom slabljenja kognitivnih sposobnosti, demencija, depresije i anksioznosti. Ciljevi ovog istraživanja bili su odrediti utjecaj starenja i šećerne bolesti tipa 1 (DM1) na neurogenezu u subventrikularnoj zoni (SVZ) mozga štakora, kao i odrediti moguću ulogu DSTYK u adultnoj neurogenezi u subventrikularnoj zoni, kao moguće mehanizme u nastanku ove bolesti. Materiali i methode: Trideset pet štakora muškog spola soja Sprague-Dawley podijeljeni su nasumično u dvije skupine: kontrolne i dijabetične životinje. DM1 je izazvan intraperitonealnom aplikacijom streptozotocina (55 mg/kg). DM1 potvrđen je mjerenjem glukoze u plazmi 4 dana nakon indukcije. Štakori su žrtvovani 2 tjedna, 6 mjeseci ili 1 godinu nakon indukcije DM1. Tkiva mozga pripremljena su te obojena imunohistokemijski na doublecortin (DCX) samostalno, ili u kombinaciji s DSTYK. Rezultati: U debljem području subependimskog sloja SVZ, postotak površine pod DCX u rezovima mozga kontrolnih štakora značajno se smanjio 6 mjeseci, u usporedbi s 2 tjedna nakon početka pokusa (P