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An automated microfluidic platform for long-term high-resolution imaging of C. elegans
- Source :
- Proceed. The 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2014), San Antonio, TX, USA.
- Publication Year :
- 2014
-
Abstract
- We describe a microfluidic platform for the automated culture, treatment and long-term highresolution imaging of Caenorhabditis elegans nematodes under normal physiological conditions. Our device features: (i) a microfluidic design tailored for the isolation of L4 larvae from a mixed larval population and for successive culture and treatment; (ii) a worm immobilization method, based on the thermo-reversible gelation of a biocompatible polymer inside the microfluidic chip, thereby enabling high-resolution imaging; (iii) an integrated temperature control system, both to ensure viable environmental conditions for C. elegans culture and to steer the worm immobilization/release process.
- Subjects :
- Pluronic F-127
fungi
C.elegans immobilization
long-term imaging
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Proceed. The 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2014), San Antonio, TX, USA
- Accession number :
- edsair.dedup.wf.001..28672af737cce188acc596e34589365a