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Analysis of mRNA and Protein Levels of CAP2, DLG1 and ADAM10 Genes in Post-Mortem Brain of Schizophrenia, Parkinson’s and Alzheimer’s Disease Patients
- Source :
- International Journal of Molecular Sciences, Vol 23, Iss 1539, p 1539 (2022)
- Publication Year :
- 2022
- Publisher :
- MDPI AG, 2022.
-
Abstract
- Schizophrenia (SCZ) is a mental illness characterized by aberrant synaptic plasticity and connectivity. A large bulk of evidence suggests genetic and functional links between postsynaptic abnormalities and SCZ. Here, we performed quantitative PCR and Western blotting analysis in the dorsolateral prefrontal cortex (DLPFC) and hippocampus of SCZ patients to investigate the mRNA and protein expression of three key spine shapers: the actin-binding protein cyclase-associated protein 2 (CAP2), the sheddase a disintegrin and metalloproteinase 10 (ADAM10), and the synapse-associated protein 97 (SAP97). Our analysis of the SCZ post-mortem brain indicated increased DLG1 mRNA in DLPFC and decreased CAP2 mRNA in the hippocampus of SCZ patients, compared to non-psychiatric control subjects, while the ADAM10 transcript was unaffected. Conversely, no differences in CAP2, SAP97, and ADAM10 protein levels were detected between SCZ and control individuals in both brain regions. To assess whether DLG1 and CAP2 transcript alterations were selective for SCZ, we also measured their expression in the superior frontal gyrus of patients affected by neurodegenerative disorders, like Parkinson’s and Alzheimer’s disease. Interestingly, also in Parkinson’s disease patients, we found a selective reduction of CAP2 mRNA levels relative to controls but unaltered protein levels. Taken together, we reported for the first time altered CAP2 expression in the brain of patients with psychiatric and neurological disorders, thus suggesting that aberrant expression of this gene may contribute to synaptic dysfunction in these neuropathologies.
- Subjects :
- Adult
Male
QH301-705.5
Discs Large Homolog 1 Protein
ADAM10 Protein
Dorsolateral Prefrontal Cortex
Hippocampu
Alzheimer Disease
Amyloid Precursor Protein Secretase
Biology (General)
Membrane Protein
QD1-999
Adaptor Proteins, Signal Transducing
Aged
Aged, 80 and over
dendritic spine
Parkinson Disease
Middle Aged
Alzheimer’s disease
Dendritic spine
Parkinson’s disease
Postsynaptic density
Schizophrenia
schizophrenia
Chemistry
Gene Expression Regulation
postsynaptic density
Female
Autopsy
Case-Control Studie
Human
Subjects
Details
- Language :
- English
- ISSN :
- 16616596 and 14220067
- Volume :
- 23
- Issue :
- 1539
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.dedup.wf.001..54f11d84baa284df393fd4be95ad6ef7