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Serological proteome analysis of Staphylococcus aureus strains isolated from gangrenous and subclinical ewe mastitis reveals core and accessory seroproteomes
- Source :
- ADSA ASAS Joint Annual Meeting, ADSA ASAS Joint Annual Meeting, Jul 2011, New Orleans, United States. Journal of Dairy Science, 94, 2011, Journal of Dairy Science, ADSA ASAS Joint Annual Meeting, Jul 2011, New Orleans, United States. 94, 2011, Journal of Dairy Science
- Publication Year :
- 2011
- Publisher :
- HAL CCSD, 2011.
-
Abstract
- Cette publication est également parue dans la revue "Journal of Animal Science", Vol.89, E-Suppl.1.; Staphylococcus aureus is a major cause of mastitis in ruminants. In ewe mastitis, symptoms range from subclinical to gangrenous mastitis. S. aureus factors or host-factors contributing to the different outcomes are not completely elucidated. In this study, experimental mastitis was induced on primiparous ewes using two S. aureus strains, isolated from gangrenous (strain O11) or subclinical (strain O46) mastitis. Strains induced drastically distinct clinical symptoms when tested in ewe and mice experimental mastitis. Notably, they reproduced mild (O46) or severe (O11) mastitis in ewes. Ewe sera were used to identify staphylococcal immunoreactive proteins commonly or differentially produced during infections of variable severity and to define core and accessory seroproteomes. Such SERological Proteome Analysis (SERPA) allowed the identification of 89 immunoreactive proteins, of which only 52 (58.4%) were previously identified as immunogenic proteins in other staphylococcal infections. Among the 89 proteins identified, 74 appear to constitute the core seroproteome. Among the 15 remaining proteins defining the accessory seroproteome, 12 were specific for strain O11, 3 were specific for O46. Distribution of one protein specific for each mastitis severity was investigated in ten other strains isolated from subclinical or clinical mastitis. We report here for the first time the identification of staphylococcal immunogenic proteins common or specific to S. aureus strains responsible for mild or severe mastitis. These findings open avenues in S. aureus mastitis studies as some of these proteins, expressed in vivo, are likely to account for the success of S. aureus as a pathogen of the ruminant mammary gland.
- Subjects :
- staphylococcus aureus
disease prevention
prévention des maladies
mammites
espèce
infection
mammite
identification d'espèce
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
protéine
[SDV.IDA]Life Sciences [q-bio]/Food engineering
identification
protein
protéome
[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- ADSA ASAS Joint Annual Meeting, ADSA ASAS Joint Annual Meeting, Jul 2011, New Orleans, United States. Journal of Dairy Science, 94, 2011, Journal of Dairy Science, ADSA ASAS Joint Annual Meeting, Jul 2011, New Orleans, United States. 94, 2011, Journal of Dairy Science
- Accession number :
- edsair.dedup.wf.001..7839a6d30f007e0d604e54a3896ff369