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USE OF CONJUGATES WITH LINKERS CLEAVABLE BY PHOTODISSOCIATION OR FRAGMENTATION FOR MASS SPECTROMETRY ANALYSIS OF TISSUE SECTIONS
- Source :
- United States, Patent n° : WO2006IB02309 20060607. 2007, pp.65
- Publication Year :
- 2007
- Publisher :
- HAL CCSD, 2007.
-
Abstract
- The invention concerns a method for determining at least one target molecule map in a tissue section, using at least one (A-X)n-B conjugate, wherein A is a tag molecule of known molecular weight, X is a linker that is cleaved during sample desorption/ionization, n is an integer of at least l,and B is a binding molecule that binds specifically to said target molecule. When using MALDI mass spectrometry, said linker molecule X may be cleaved by photodissociation during sample laser irradiation if photocleavable at the wavelength of said MALDI laser. Alternatively, when using UV- MALDI, IR-MALDI, SIMS or DESI mass spectrometry, said linker molecule X may be cleaved by fragmentation during sample desorption/ionization.
- Subjects :
- MALDI imaging
diagnosis
[CHIM.ANAL]Chemical Sciences/Analytical chemistry
specific imaging
mass sepctrometry
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
[SDV.CAN]Life Sciences [q-bio]/Cancer
[SDV.IB]Life Sciences [q-bio]/Bioengineering
Tag mass
[INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM]
[SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences
[SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM]
[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- United States, Patent n° : WO2006IB02309 20060607. 2007, pp.65
- Accession number :
- edsair.dedup.wf.001..899522d49a34216ffb0f73ebcbf3b8d6