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Crosslinked fibrin gels for tissue engineering: Two approaches to improve their properties
- Source :
- RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia, instname
- Publication Year :
- 2015
- Publisher :
- John Wiley & Sons, 2015.
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Abstract
- [EN] Fibrin is a protein that can be used as an ideal scaffolding material to promote tissue regeneration. In order to enhance its physical properties in this study a natural crosslinker, genipin (GP), was employed with the aim to obtain a hydrogel with tuneable properties for tissue engineering applications. The fibrin gel was crosslinked by two different methods using four concentrations of GP to get a stable hydrogel network. Crosslinking density, mechanical properties, swelling, and enzymatic degradation of the hydrogels were tested for each GP content and method employed. The method I: Crosslinking after gel formation promotes a high crosslinking and retains the gel shape for long term whilst the method II: Simultaneous gel formation and crosslinking improves the mechanical properties of the gel. This study confirms the use of GP at different concentrations as a suitable crosslinker of fibrin that promotes the cellular viability of L929 for 21 days of in vitro culture.<br />Contract grant sponsor: Ciber-BBN, Instituto de Salud Carlos III, European Regional Development Fund
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia, instname
- Accession number :
- edsair.dedup.wf.001..8ed1843c69dac231d9604b920f7f371e