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The Cheese Matrix Modulates the Immunomodulatory Properties of Propionibacterium freudenreichii CIRM-BIA 129 in Healthy Piglets
- Source :
- Frontiers in Microbiology, Frontiers in Microbiology, Frontiers Media, 2018, 9, pp.1-15. ⟨10.3389/fmicb.2018.02584⟩, Frontiers in Microbiology (9), 1-15. (2018), Frontiers in Microbiology, 2018, 9, pp.1-15. ⟨10.3389/fmicb.2018.02584⟩
- Publication Year :
- 2018
- Publisher :
- Frontiers Media SA, 2018.
-
Abstract
- International audience; Propionibacterium freudenreichii is a beneficial bacterium, used as a cheese starter, which presents versatile probiotic properties. These properties are strain-dependent. We hypothesized they may also be delivery vehicle-dependent. In this study, we thus explored in healthy piglets how the cheese matrix affects the immunomodulatory properties of P. freudenreichii. During 2 weeks, three groups of weaned piglets consumed, respectively, P. freudenreichii as a liquid culture (PF-culture), P. freudenreichii under the form of a cheese (PF-cheese), or a control sterile cheese matrix (Cheesematrix). The in vivo metabolic activity of P. freudenreichii was assessed by determining short chain fatty acids (SCFA) concentration and bifidobacteria population in feces. Whatever the delivery vehicle, P. freudenreichii was metabolically active in piglets’ colon and enhanced both bifidobacteria and SCFA in feces. P. freudenreichii consumption decreased the secretion of TNFa and of IL-10 by peripheral blood mononuclear cells (PBMC). It did not alter IL-10, IFNg, IL-17, and TNFa secretion in mesenteric lymph node immune cells (MLNC). PF-cheese enhanced significantly Treg phenotype, while PF-culture decreased significantly Th17 phenotype in PBMC and MLNC. Remarkably, only PF-cheese induced an increase of Th2 phenotype in PBMC and MLNC. Ex vivo stimulation of PBMC and MLNC by Lipopolysaccharides and Concanavalin A emphasized the difference in the immunomodulatory responses between PF-culture and PF-cheese group, as well as between PBMC and MLNC. This study shows the importance to consider the delivery vehicle for probiotic administration. It confirms the anti-inflammatory potential of P. freudenreichii. It opens new perspectives for the use propionibacteria-fermented products as preventive agents for inflammatory bowel diseases and intestinal infectious diseases.
- Subjects :
- Microbiology (medical)
modèle animal
matrice fromagère
Ingénierie des aliments
human health
immunomodulation
Microbiology
[SDV.IDA]Life Sciences [q-bio]/Food engineering
Food and Nutrition
Food engineering
[SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology
probiotique
propionibacterium freudenreichii
T lymphocytes phenotype
PBMC
MLNCv
inflammation de l'intestin
ComputingMilieux_MISCELLANEOUS
Microbiology and Parasitology
food and beverages
santé humaine
[SDV.IDA] Life Sciences [q-bio]/Food engineering
Microbiologie et Parasitologie
animal models
[SDV.AEN] Life Sciences [q-bio]/Food and Nutrition
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Alimentation et Nutrition
[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
probiotic
Subjects
Details
- Language :
- English
- ISSN :
- 1664302X
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Frontiers in Microbiology
- Accession number :
- edsair.dedup.wf.001..95239edead832bf088fbefb930ed45fe
- Full Text :
- https://doi.org/10.3389/fmicb.2018.02584