Back to Search
Start Over
alpha v integrin processing interferes with the cross-talk between alpha v beta 5/beta 6 and alpha 2 beta 1 integrins
- Source :
- Biology of the Cell, Biology of the Cell, Wiley, 2011, 103 (11), pp.519-529. ⟨10.1042/BC20100147⟩
- Publication Year :
- 2011
- Publisher :
- HAL CCSD, 2011.
-
Abstract
- International audience; Background information. Previous studies have reported that cross-talk between integrins may be an important regulator of integrin-ligand binding and subsequent signalling events that control a variety of cell functions in many tissues. We previously demonstrated that alpha v beta 5/beta 6 integrin represses alpha 2 beta 1-dependent cell migration. The alpha v subunits undergo an endoproteolytic cleavage by protein convertases, whose role in tumoral invasion has remained controversial. Results. Inhibition of convertases by the convertase inhibitor alpha 1-PDX (alpha 1-antitrypsin Portland variant), leading to the cell-surface expression of an uncleaved form of the av integrin, stimulated cell migration toward type I collagen. Under convertase inhibition, alpha 2 beta 1 engagement led to enhanced phosphorylation of both FAK (focal adhesion kinase) and MAPK (mitogen-activated protein kinase). This outside-in signalling stimulation was associated with increased levels of activated integrin located in larger than usual focal-adhesion structures and a cell migration that was independent of the PI3K (phosphoinositide 3-kinase)/Akt (also called protein kinase B) pathway. Conclusions. The increase in cell migration observed upon convertases inhibition appears to be due to the up-regulation of beta 1 integrins and to their location in larger focal-adhesion structures. The endoproteolytic cleavage of av subunits is necessary for alpha v beta 5/beta 6 integrin to control alpha 2 beta 1 function and could thus play an essential role in colon cancer cell migration.
Details
- Language :
- English
- ISSN :
- 02484900 and 1768322X
- Database :
- OpenAIRE
- Journal :
- Biology of the Cell, Biology of the Cell, Wiley, 2011, 103 (11), pp.519-529. ⟨10.1042/BC20100147⟩
- Accession number :
- edsair.dedup.wf.001..9669c8457c41d1f8faf0beb89ab58c4d