The influence of training of different intensities on the incidence of inflammatory and antiinflamatory marker in amateur athletes

Cilj istraživanja: Cilj ovog istraživanja je utvrditi kako trening različitog intenziteta (ispod ili iznad bazalnog metabolizma) utječe na monocitne subpopulacije na uzorku sportaša rekreativaca. Materijali i metode: Uzorci krvi svih sudionika uzeti su za analizu 30 minuta prije i nakon treninga. Uzorak je tretiran reagensom za blokiranje Fc receptora te je inkubirana 20 minuta u mraku na 25°C s 4 μL anti-humanih CD14s PerCP-Cy5.5 konjugiranih antitijela i 5 μL fikoeritrinom konjugiranih antitijela reaktivnih na ljudski CD16. Nakon lize crvenih krvnih stanica otopinom za lizu, stanice su analizirane na protočnom citometru. Mjereni su također i uzorci neobojanih stanica i obrađeni su kao negativne kontrole. Podaci prikupljeni citometrom analizirani su pomoću softvera FlowLogic. Rezultati: Kao rezultat istraživanja dobili smo podatak da tjelesna aktivnost ima utjecaj na aktivaciju protuupalne te upalne monocitne skupine. Povećanjem intenziteta tjelesne aktivnosti opažen je porast broja monocitnih skupina. Također rezultati govore da vrijeme uzorkovanja krvi i broj ispitanika utječu na rezultate. Zaključak: Trening različitog intenziteta kod sportaša rekreativaca (ispod ili iznad bazalnog metabolizma) utjecao je na porast broja upalnih i protuupalnih monocita, ali je za bolje razumijevanje utjecaja intenziteta potrebno uzeti u obzir vrijeme uzorkovanja krvi i veći uzorak.
The aim of this study: The aim of this research is to determine how training at different intensities (below or above basal metabolism) affects monocyte subpopulations in a sample of recreational athletes. Methods: Blood samples of all participants were taken for analysis 30 minutes before and after training. The sample was treated with Fc receptor blocking reagent and incubated for 20 minutes in the dark at 25°C with 4 μL of anti-human CD14s PerCP-Cy5.5 conjugated antibodies and 5 μL of phycoerythrin-conjugated antibodies reactive to human CD16. After the lysis of red blood cells with lysis solution, the cells were analyzed on a flow cytometer. Samples of unstained cells were also measured and processed as negative controls. Data collected by cytometer were analyzed using FlowLogic software. Results: As a result of the research, we obtained an information that physical activity has an impact on the activation of anti-inflammatory and inflammatory monocyte groups. By increasing the intensity of physical activity, an increase in the number of monocyte groups was observed. The results also tell that the time of blood sampling and the number of subjects have an influence on results. Conclusion: Training of different intensity in recreational athletes (below or above basal metabolism) affects the increase in the number of inflammatory and anti-inflammatory monocytes, but for a better understanding of intensity influence, it is necessary to take into account the time of blood sampling and a larger sample.