The primary photophysics of the Avena sativa phototropin 1 LOV2 domain observed with time-resolved emission spectroscopy

Authors :: van Stokkum, I.H.M.
Gauden, M.
Crosson, S.
van Grondelle, R.
Moffat, K.
Kennis, J.T.M.
Biophysics Photosynthesis/Energy
LaserLaB - Energy
Source :: van Stokkum, I H M, Gauden, M, Crosson, S, van Grondelle, R, Moffat, K & Kennis, J T M 2011, ' The primary photophysics of the Avena sativa phototropin 1 LOV2 domain observed with time-resolved emission spectroscopy ', Photochemistry and Photobiology, vol. 87, no. 3, pp. 534-541 . https://doi.org/10.1111/j.1751-1097.2011.00903.x, Photochemistry and Photobiology, 87(3), 534-541. Wiley-Blackwell
Publication Year :: 2011
Abstract: The phototropins are blue-light receptors that base their light-dependent action on the reversible formation of a covalent bond between a flavin mononucleotide (FMN) cofactor and a conserved cysteine in light, oxygen or voltage (LOV) domains. The primary reactions of the Avena sativa phototropin 1 LOV2 domain were investigated by means of time-resolved and low-temperature fluorescence spectroscopy. Synchroscan streak camera experiments revealed a fluorescence lifetime of 2.2 ns in LOV2. A weak long-lived component with emission intensity from 600 to 650 nm was assigned to phosphorescence from the reactive FMN triplet state. This observation allowed determination of the LOV2 triplet state energy level at physiological temperature at 16600 cm

Language :: English
ISSN :: 00318655
Database :: OpenAIRE
Journal :: van Stokkum, I H M, Gauden, M, Crosson, S, van Grondelle, R, Moffat, K & Kennis, J T M 2011, ' The primary photophysics of the Avena sativa phototropin 1 LOV2 domain observed with time-resolved emission spectroscopy ', Photochemistry and Photobiology, vol. 87, no. 3, pp. 534-541 . https://doi.org/10.1111/j.1751-1097.2011.00903.x, Photochemistry and Photobiology, 87(3), 534-541. Wiley-Blackwell
Accession number :: edsair.dedup.wf.001..a6215e23817d852c2bd9c1ef17e983db
Full Text :: https://doi.org/10.1111/j.1751-1097.2011.00903.x

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