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Enzymatic and non-enzymatic protective mechanisms in recalcitrant seeds of Araucaria bidwillii subjected to desiccation

Enzymatic and non-enzymatic protective mechanisms in recalcitrant seeds of Araucaria bidwillii subjected to desiccation

Authors :
Francini, A
Galleschi, L
Saviozzi, F
Pinzino, C
Izzo, Riccardo
Sgherri, Cristina
Navari, Flavia
Source :
Plant physiology and biochemistry (Paris) 44 (2006): 556–563., info:cnr-pdr/source/autori:A.Francini, L. Galleschi, F. Saviozzi, C. Pinzino, R. Izzo, C. L. M. Sgherri, F. Navari-Izzo/titolo:Enzymatic and non-enzymatic protective mechanisms in recalcitrant seeds of Araucaria bidwillii subjected to desiccation/doi:/rivista:Plant physiology and biochemistry (Paris)/anno:2006/pagina_da:556/pagina_a:563/intervallo_pagine:556–563/volume:44
Publication Year :
2006
Publisher :
Elsevier, Montrouge , Francia, 2006.

Abstract

The changes in several antioxidants as well as in the level of C-centered free radicals and thiobarbituric acid reactive substances (TBARS) were studied in seeds of Araucaria bidwillii Hook desiccated to 37%, 28% and 21% moisture content. The lowest-safe moisture content for the seedling establishment was 37%. The embryo, besides double amounts of free radicals, showed higher levels of both enzymatic and nonenzymatic antioxidants than endosperm. Lutein decreased in both organs whereas á-tocopherol values were not affected by desiccation. In the embryo at 37% seed moisture content the antioxidant defense system increased giving rise to a decrease in free radicals. Beyond this point, free radicals and TBARS increased in agreement with the umpiring of the ascorbate/glutathione cycle by the decrease in reduced glutathione and glutathione reductase activity (GR, EC 1.6.4.2). At 21% moisture GR decreased. In the endosperm during desiccation, the consumption of ascorbate, total glutathione and lutein prevented the rise in free radicals and TBARS till 28% moisture, at which an increase in oxidized glutathione was also observed.

Details

Language :
English
Database :
OpenAIRE
Journal :
Plant physiology and biochemistry (Paris) 44 (2006): 556–563., info:cnr-pdr/source/autori:A.Francini, L. Galleschi, F. Saviozzi, C. Pinzino, R. Izzo, C. L. M. Sgherri, F. Navari-Izzo/titolo:Enzymatic and non-enzymatic protective mechanisms in recalcitrant seeds of Araucaria bidwillii subjected to desiccation/doi:/rivista:Plant physiology and biochemistry (Paris)/anno:2006/pagina_da:556/pagina_a:563/intervallo_pagine:556–563/volume:44
Accession number :
edsair.dedup.wf.001..b2569d2a4b47a06c7c2a746b13d037e2