Back to Search
Start Over
Bacteriophage Sf6 Tailspike Protein for Detection of Shigella flexneri Pathogens
- Source :
- Viruses, Viruses, MDPI, 2018, 10 (8), pp.431. ⟨10.3390/v10080431⟩, Viruses, Vol 10, Iss 8, p 431 (2018), Viruses, 2018, 10 (8), pp.431. ⟨10.3390/v10080431⟩
- Publication Year :
- 2018
- Publisher :
- HAL CCSD, 2018.
-
Abstract
- International audience; Bacteriophage research is gaining more importance due to increasing antibiotic resistance. However, for treatment with bacteriophages, diagnostics have to be improved. Bacteriophages carry adhesion proteins, which bind to the bacterial cell surface, for example tailspike proteins (TSP) for specific recognition of bacterial O-antigen polysaccharide. TSP are highly stable proteins and thus might be suitable components for the integration into diagnostic tools. We used the TSP of bacteriophage Sf6 to establish two applications for detecting Shigella flexneri (S. flexneri), a highly contagious pathogen causing dysentery. We found that Sf6TSP not only bound O-antigen of S. flexneri serotype Y, but also the glucosylated O-antigen of serotype 2a. Moreover, mass spectrometry glycan analyses showed that Sf6TSP tolerated various O-acetyl modifications on these O-antigens. We established a microtiter plate-based ELISA like tailspike adsorption assay (ELITA) using a Strep-tag®II modified Sf6TSP. As sensitive screening alternative we produced a fluorescently labeled Sf6TSP via coupling to an environment sensitive dye. Binding of this probe to the S. flexneri O-antigen Y elicited a fluorescence intensity increase of 80% with an emission maximum in the visible light range. The Sf6TSP probes thus offer a promising route to a highly specific and sensitive bacteriophage TSP-based Shigella detection system.
- Subjects :
- MESH: Gene Expression
MESH: Shigella flexneri
[SDV]Life Sciences [q-bio]
lcsh:QR1-502
MESH: Protein Structure, Secondary
MESH: Biological Assay
lcsh:Microbiology
MESH: Bacterial Typing Techniques
Shigella flexneri
tailspike proteins
fluorescence sensor
MESH: Recombinant Proteins
ddc:05
bacteriophage
MESH: Genetic Vectors
[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases
MESH: Protein Binding
MESH: Cloning, Molecular
ddc:610
MESH: Bacteriophages
MESH: Viral Tail Proteins
Institut für Biochemie und Biologie
microtiter plate assay
MESH: O Antigens
MESH: Carbohydrate Sequence
MESH: Escherichia coli
MESH: Oxadiazoles
MESH: Enzyme-Linked Immunosorbent Assay
MESH: Podoviridae
MESH: Serogroup
O-antigen
MESH: Fluorescent Dyes
serotyping
[SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics
MESH: Staining and Labeling
610 Medizin und Gesundheit
MESH: Models, Molecular
Subjects
Details
- Language :
- English
- ISSN :
- 19994915
- Database :
- OpenAIRE
- Journal :
- Viruses, Viruses, MDPI, 2018, 10 (8), pp.431. ⟨10.3390/v10080431⟩, Viruses, Vol 10, Iss 8, p 431 (2018), Viruses, 2018, 10 (8), pp.431. ⟨10.3390/v10080431⟩
- Accession number :
- edsair.dedup.wf.001..b73ac4f22e36f25a48e566dca1a5cb4b