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Evolution of the primate beta-globin gene region. High rate of variation in CpG dinucleotides and in short repeated sequences between man and chimpanzee
- Source :
- Journal of Molecular Biology, Journal of Molecular Biology, Elsevier, 1985, 182 (1), pp.21-29. ⟨10.1016/0022-2836(85)90024-5⟩, Journal of Molecular Biology, 1985, 182 (1), pp.21-29. ⟨10.1016/0022-2836(85)90024-5⟩
- Publication Year :
- 1985
- Publisher :
- HAL CCSD, 1985.
-
Abstract
- A 5500 base-pair fragment including the β-globin gene downstream from codon 122 and about 4000 base-pairs of its 5′ flanking sequence was cloned from chimpanzee DNA and thoroughly sequenced before being compared with the corresponding human sequence: 88 point differences (83 substitutions and 5 deletions or insertions of 1 base-pair) were detected as well as seven more important deletion/insertion events. These changes occur preferentially in two kinds of structure. First, 40% of the CpG dinucleotides present in either human or chimpanzee sequences are affected by nucleotide variations. This corresponds to a divergence level considerably higher than that expected. Second, most short repeated sequences found in the 5′ extragenic sequence are involved in mutational events (amplification or contraction of the number of basic motifs as well as point substitutions or deletions/insertions of 1 base-pair). Considering the very low level of nucleotide sequence divergence between these two closely related species, our data provide direct evidence for CpG and tandem array instability.
- Subjects :
- [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT]
Subjects
Details
- Language :
- English
- ISSN :
- 00222836 and 10898638
- Database :
- OpenAIRE
- Journal :
- Journal of Molecular Biology, Journal of Molecular Biology, Elsevier, 1985, 182 (1), pp.21-29. ⟨10.1016/0022-2836(85)90024-5⟩, Journal of Molecular Biology, 1985, 182 (1), pp.21-29. ⟨10.1016/0022-2836(85)90024-5⟩
- Accession number :
- edsair.dedup.wf.001..f0d8de756be0904a454641abc1e93945