Studies on the antioxidant effect of hydrogen microbial preparation on acute hypoxia rats

Objective To explore the antioxidant effect of hydrogen bacterial preparation on acute hypoxia rats. Methods Forty Sprague-Dawley rats were designed to 5 groups (8 each): normoxic saline group (NS), high altitude saline group (HS), high altitude low-dose group (HL), high altitude medium-dose group (HM) and high altitude high-dose group (HH) hydrogen bacterial preparation (10, 20 and 40mg/kg, respectively). Rats in NS group and HS group were given 2ml of normal saline by intragastric administration, and in the rest 3 plateau groups were intragastrically administered with preparations of corresponding dosage at a simulated altitude of 5000m once a day for 3 days. Contents of tumor necrosis factor alpha (TNF-α) in plasma, malondialdehyde (MDA), catalase (CAT), superoxide dismutase (SOD) and glutathione (GSH) in liver, lung and heart were then measured. Results Compared with HS group, the content of plasma TNF-α in HL group was significantly decreased (P