Effects of telmisartan on the expression of adiponectin receptor 1 in cardiomyocytes and its mechanism

Objective To explore the mechanisms of telmisartan in regulating the expression of adiponectin receptor 1 (AdipoR1) in diabetic cardiomyocytes. Methods Investigation of the inhibitory effects of AngⅡ were performed in 3 subexperiments as follows: One detects mRNA and protein expression of AdipoR1 in different batches of H9C2 cells pre-treated with AngⅡ for 48 hours at a concentration of 0, 10-8, 10-7, 10-6, 10-5mol/L respectively, one in cells pre-treated with AngⅡ at a concentration of 10-7mol/L for 0, 12, 24, 36 and 48 hours respectively, while the last in cells pre-treated with telmisartan at 10-5mol/L for 1 hour followed by AngⅡ at 10-7mol/L for 24 hours. Cultured H9C2 cardiomyocytes were randomly divided into following five groups: normal control group (NG), high glucose group (HG), high glucose plus telmisartan group (HG+T), high glucose plus telmisartan and PPAR-γ antagonist GW9662 group (HG+T+GW), and normal glucose plus mannitol group (NG+M). Cardiomyocytes in the above-mentioned five groups were continued to be cultured for 24h, then the mRNA and protein expressions of AdipoR1 in cardiomyocytes were assayed. Fluorescent quantitative real-time PCR and Western blotting were used respectively to analyze the mRNA and protein expressions of AdipoR1 in cardiomyocytes. Results The mRNA and protein expressions of AdipoR1 in cardiomyocytes were significantly decreased by AngⅡ at concentrations of 10-8, 10-7, 10-6 and 10-5mol/L (P0.05). The mRNA and protein expressions of AdipoR1 in cardiomyocytes were significantly up-regulated in group HG+T compared with that in group HG (P