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Suppression of OCT4-pg5 and OCT4B enhanced the sensitivity of bladder cancer cells T24 to cisplatin
- Source :
- Medical Journal of Chinese People's Liberation Army, Vol 45, Iss 2, Pp 176-181 (2020)
- Publication Year :
- 2020
- Publisher :
- Editorial Board of Medical Journal of Chinese People's Liberation Army, 2020.
-
Abstract
- Objective To investigate the effects of octamer-binding transcription factor 4 pseudogene 5 (OCT4-pg5) and octamer-binding transcription factor 4B (OCT4B) expression on cisplatin sensitivity of bladder cancer T24 cells. Methods The expression of OCT4-pg5 and OCT4B in bladder cancer cells and tissues were quantified by real-time quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR). To examine the effect of OCT4-pg5 and OCT4B on cisplatin sensitivity, cells were transfected with OCT4-pg5 si-RNA, OCT4B si-RNA and their control si-RNA by lipofectamine 2000 followed by a 48 h cisplatin treatment. CCK8 was used to assess the half-maximal inhibitory concentration (IC50) of cisplatin in T24 cells. OCT4-pg5 and OCT4B expression were detected by RT-qPCR or Western blot. Next, flow cytometry was used to examine the effects of OCT4-pg5 and OCT4B on T24 cells apoptosis and cell-cycle distribution. Results RT-qPCR results showed that OCT4-pg5 and OCT4B were highly expressed in bladder cancer cells and tissues. Moreover, OCT4-pg5 expression was positively correlated with OCT4B expression in 23 bladder cancer tissues. Cells transfected with OCT4-pg5 si-RNA or OCT4B si-RNA showed a much lower IC50 of cisplatin, compared with cells in the control group. RT-qPCR or Western blot results showed OCT4-pg5 or OCT4B expression was significantly down-regulated after being transfected with OCT4-pg5 si-RNA or OCT4B si-RNA in cisplatin treated T24 cells. The flow cytometry results showed that OCT4-pg5 suppression or OCT4B suppression of cisplatin treated T24 cells resulted in a significant increase of cell apoptosis and marked the transition from the S phase to G0/G1 phase. Conclusions Downregulating OCT4-pg5 and OCT4B could enhance the sensitivity of bladder cancer T24 cells to cisplatin. DOI: 10.11855/j.issn.0577-7402.2020.02.11
Details
- Language :
- Chinese
- ISSN :
- 05777402
- Volume :
- 45
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Medical Journal of Chinese People's Liberation Army
- Accession number :
- edsair.doajarticles..8409fe5957b188ac5ea5f95b4752e5d9