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A T-Cell Surface Marker Panel Predicts Murine Acute Graft-Versus-Host Disease

Authors :
Carina A. Bäuerlein
Musga Qureischi
Zeinab Mokhtari
Paula Tabares
Christian Brede
Ana-Laura Jordán Garrote
Simone S. Riedel
Martin Chopra
Simone Reu
Anja Mottok
Estibaliz Arellano-Viera
Carolin Graf
Miriam Kurzwart
Katharina Schmiedgen
Hermann Einsele
Matthias Wölfl
Paul-Gerhardt Schlegel
Andreas Beilhack
Source :
Frontiers in Immunology, Vol 11 (2021)
Publication Year :
2021
Publisher :
Frontiers Media S.A., 2021.

Abstract

Acute graft-versus-host disease (aGvHD) is a severe and often life-threatening complication of allogeneic hematopoietic cell transplantation (allo-HCT). AGvHD is mediated by alloreactive donor T-cells targeting predominantly the gastrointestinal tract, liver, and skin. Recent work in mice and patients undergoing allo-HCT showed that alloreactive T-cells can be identified by the expression of α4β7 integrin on T-cells even before manifestation of an aGvHD. Here, we investigated whether the detection of a combination of the expression of T-cell surface markers on peripheral blood (PB) CD8+ T-cells would improve the ability to predict aGvHD. To this end, we employed two independent preclinical models of minor histocompatibility antigen mismatched allo-HCT following myeloablative conditioning. Expression profiles of integrins, selectins, chemokine receptors, and activation markers of PB donor T-cells were measured with multiparameter flow cytometry at multiple time points before the onset of clinical aGvHD symptoms. In both allo-HCT models, we demonstrated a significant upregulation of α4β7 integrin, CD162E, CD162P, and conversely, a downregulation of CD62L on donor T-cells, which could be correlated with the development of aGvHD. Other surface markers, such as CD25, CD69, and CC-chemokine receptors were not found to be predictive markers. Based on these preclinical data from mouse models, we propose a surface marker panel on peripheral blood T-cells after allo-HCT combining α4β7 integrin with CD62L, CD162E, and CD162P (cutaneous lymphocyte antigens, CLA, in humans) to identify patients at risk for developing aGvHD early after allo-HCT.

Details

Language :
English
ISSN :
16643224
Volume :
11
Database :
OpenAIRE
Journal :
Frontiers in Immunology
Accession number :
edsair.doajarticles..9f2aa6e4d7a8cd15196534de3f77905a
Full Text :
https://doi.org/10.3389/fimmu.2020.593321/full