Expression of c-myc gene and biosynthesis of biological macromolecules in antisense transfectant HL60 R-9

The recombinant plasmid PGC was constructed for transcription unit of c-myc gene with diorientation in vitro , to make RNA probes for detection of c-myc mRNA and antisence RNA expression of tranfectant HL R 60 -9, which was obtained from HL60 cells transfected with inducible c-myc antisense RNA expression plasmid. The results from HL R 60 -9 cells induced by Cd 2+ indicated that expression of c-myc antisense RNA increased with Cd 2+ concentration and exposure time, while c-myc mRNA expression progressively reduced. Using immunohistochemical technique no c-myc P62 protein expression was detected. The incorporation of 3 H-TdR, 3 H-UR and 3 HLeu revealed significant suppression of DNA, RNA and protein biosynthesis. It is suggested that the reversion changes previously reported in malignant phenotypes of HL R 60 -9 cells and the inhibition of macromolecular biosynthesis mentioned above were associated with the blockade of c-myc gene expression by its antisense RNA.