TISSUE CULTURE OF MICE KIDNEYS FOR THE TOXICITY EVALUATION OF SOME CYTOSTATICS

The rationale for the present study is explained by a high risk of the development of side effects, including nephrotoxic, in patients that receive the treatment with cytostatics bleocin, lastet, and cisplatin for oncologic diseases. The present article focuses on the evaluation of the toxicity of the mentioned drugs. The method of plasma-free renal tissue cultures in mice on the perforated millipore filters was used. The identification of the toxicity of different doses of the drugs was performed by the comparison of the areas of the grown colonies. The authors presented the data that the inhibition of the growth of renal tissue cultures (by 50%) required a 4.3-fold increase in the dose of lastet in comparison with bleocin. The inhibition of the tissue culture colony growth with cisplatin by 85% was registered in the dose of the drug that corresponded to a 3% solution. The materials of the article have significant practical value because a rational indication of cytostatics bleocin, lastet, and cisplatin with regard to their nephrotoxic effect will allow the specialists to improve the treatment outcome and will contribute to the improvement of the quality of life of patients.