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Collagen/hydroxyapatite bone grafts manufactured by homogeneous/heterogeneous 3D printing
- Source :
- Materials Letters. 231:179-182
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- This paper presents a new way to obtain collagen/hydroxyapatite (COLL/HA) composite materials by 3D printing. Because of high tendency of segregation of COLL/HA composite materials, printing was done using COLL/Ca2+ gel (even COLL/Ca(OH)2) followed by precipitation of HA and crosslinking of COLL. The HA precipitation occurs simultaneously with crosslinking of COLL molecules, these processes being assured by the presence of glutaraldehyde supplemented PBS solution. By printing with COLL/Ca2+ at acidic pH homogeneity was increased. FTIR spectroscopy and microscopy reveal HA formation as the main inorganic phase these nanoparticles being homogeneously dispersed in the volume. In vitro biocompatibility assays were performed on Vero cells (ISO10993-5/2009). Results show that the extract of the developed biomaterial in cell culture medium elicited a similar cellular response to standard culture medium demonstrating its potential for biomedical applications. The described procedure and proposed ink can be used for developing 3D bone scaffolds and open new perspectives for COLL/Ca2+ homogeneous inks.
- Subjects :
- Materials science
Mechanical Engineering
Biomaterial
Nanoparticle
02 engineering and technology
010402 general chemistry
021001 nanoscience & nanotechnology
Condensed Matter Physics
In vitro biocompatibility
01 natural sciences
0104 chemical sciences
chemistry.chemical_compound
Chemical engineering
chemistry
Mechanics of Materials
Homogeneous
General Materials Science
Glutaraldehyde
Fourier transform infrared spectroscopy
0210 nano-technology
Subjects
Details
- ISSN :
- 0167577X
- Volume :
- 231
- Database :
- OpenAIRE
- Journal :
- Materials Letters
- Accession number :
- edsair.doi...........02c0929fe369ab7ec4d4267aa817f2b0