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Green synthesis and characterization of silver (Ag) nanoparticles using neem leaf extract and its antifungal activity against seed borne pathogens in chilli
- Source :
- THE ASIAN JOURNAL OF HORTICULTURE. 11:109-113
- Publication Year :
- 2016
- Publisher :
- Hind Agri Horticultural Society, 2016.
-
Abstract
- The silver nanoparticles (AgNPs) synthesized using hot water neen leaf extract (NLF) as reducing and stabilizing agent are reported and evaluated for antibacterial activity against chilli seed borne pathogens. The effect of extract concentration, contact time, pH and temperature on the reaction rate and the shape of the Ag nanoparticles were investigated. The data revealed that the rate of formation of the nanosilver size decreased significantly in the basic medium with different concentration (1 mM, 5 mM and 10 mM). Synthesized was characterized by UV-vis spectroscopy, X-ray diffraction, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The nanoparticle synthesis using different concentration of silver nitrate and 5 mM was getting nano sized silver particles AgNPs. The silver nanoparticles were with an average size of 55 to 350 nm and mostly rod shape. The antifungal activity of synthesized AgNPs was observed in seed health test and different concentration of silver NPs (750 mg, 1000 mg and 1250 mg) were used for seed health test. The AgNPs at 750 mg concentration significantly inhibited the seed borne pothogens. Thus AgNPs showed broad spectrum antifungal activity at lower concentration and may be a good alternative therapeutic approach in future.
- Subjects :
- 0106 biological sciences
Antifungal
030504 nursing
Scanning electron microscope
medicine.drug_class
Nanoparticle
01 natural sciences
Silver nanoparticle
Reaction rate
03 medical and health sciences
Silver nitrate
chemistry.chemical_compound
chemistry
Transmission electron microscopy
Botany
medicine
0305 other medical science
Antibacterial activity
010606 plant biology & botany
Nuclear chemistry
Subjects
Details
- ISSN :
- 0976724X and 09734767
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- THE ASIAN JOURNAL OF HORTICULTURE
- Accession number :
- edsair.doi...........02c53ede075c4b2fe713fb8dcde7c663