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Microfluidic Platform for Single Nucleotide Polymorphism Genotyping of the Thiopurine S-Methyltransferase Gene to Evaluate Risk for Adverse Drug Events

Authors :
Jeeshan Chowdhury
Christopher J. Backhouse
Alexey Atrazhev
Jana Lauzon
Linda M. Pilarski
Alistair J Makin
Alex Stickel
Govind V. Kagiala
Sudeep Pushpakom
William G. Newman
Source :
The Journal of Molecular Diagnostics. 9:521-529
Publication Year :
2007
Publisher :
Elsevier BV, 2007.

Abstract

Prospective clinical pharmacogenetic testing of the thiopurine S-methyltransferase gene remains to be realized despite the large body of evidence demonstrating clinical benefit for the patient and cost effectiveness for health care systems. We describe an entirely microchip-based method to genotype for common single nucleotide polymorphisms in the thiopurine S-methyltransferase gene that lead to serious adverse drug reactions for patients undergoing thiopurine therapy. Restriction fragment length polymorphism and allele-specific polymerase chain reaction have been adapted to a microfluidic chip-based polymerase chain reaction and capillary electrophoresis platform to genotype the common *2, *3A, and *3C functional alleles. In total, 80 patients being treated with thiopurines were genotyped, with 100% concordance between microchip and conventional methods. This is the first report of single nucleotide polymorphism detection using portable instrumentation and represents a significant step toward miniaturized for personalized treatment and automated point-of-care testing.

Details

ISSN :
15251578
Volume :
9
Database :
OpenAIRE
Journal :
The Journal of Molecular Diagnostics
Accession number :
edsair.doi...........035c73a933c95d8d08e20e7badc92158