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ATG5 suppresses type I IFN-dependent neutrophil swarming and NETosis
- Publication Year :
- 2023
- Publisher :
- Cold Spring Harbor Laboratory, 2023.
-
Abstract
- SUMMARYInflammation is critical for controlling infections, but when left unchecked can cause tissue damage and disease. For tuberculosis, the leading cause of death due to infection1, host inflammation is responsible for the clinical symptoms2, morbidity2, and mortality3,4. Specifically, neutrophil-dominated inflammation is associated with tuberculosis disease progression3,5,6. Therefore, understanding how neutrophil functions are regulated during infection is important for developing ways to prevent disease.Atg5was the first gene shown to specifically function within neutrophils to promote control ofMycobacterium tuberculosis7, the causative agent of tuberculosis. ATG5 is best studied for its role in autophagy8–11, however, the protective activity of ATG5 in neutrophils was unexpectedly independent of other autophagy proteins and remained elusive7. We report here that ATG5, but not other autophagy proteins, is required in neutrophils to suppress neutrophil NETosis and swarming that occur due to elevated type I interferon levels duringM. tuberculosisinfection. The elevated level of NETosis that results from loss of ATG5 expression contributes to the early susceptibility ofAtg5fl/fl-LysM-Cremice duringM. tuberculosisinfection. NETosis is associated with poor disease outcomes in tuberculosis12,13and COVID-19 patients14,15, as well as during other inflammatory diseases in humans16,17. Our studies identify an essential regulator of NETosis and elucidate previously unappreciated roles for ATG5 during infection, which may inform the design of host-directed therapeutics modulating these pathways.
Details
- Database :
- OpenAIRE
- Accession number :
- edsair.doi...........0473fcfc8e1230667910377f692aa0a9