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Postremission sequential monitoring of minimal residual disease by <scp>WT</scp> 1 Q‐ <scp>PCR</scp> and multiparametric flow cytometry assessment predicts relapse and may help to address risk‐adapted therapy in acute myeloid leukemia patients
- Source :
- Cancer Medicine. 5:265-274
- Publication Year :
- 2015
- Publisher :
- Wiley, 2015.
-
Abstract
- Risk stratification in acute myeloid leukemia (AML) patients using prognostic parameters at diagnosis is effective, but may be significantly improved by the use of on treatment parameters which better define the actual sensitivity to therapy in the single patient. Minimal residual disease (MRD) monitoring has been demonstrated crucial for the identification of AML patients at high risk of relapse, but the best method and timing of MRD detection are still discussed. Thus, we retrospectively analyzed 104 newly diagnosed AML patients, consecutively treated and monitored by quantitative polymerase chain reactions (Q-PCR) on WT1 and by multiparametric flow cytometry (MFC) on leukemia-associated immunophenotypes (LAIPs) at baseline, after induction, after 1st consolidation and after 1st intensification. By multivariate analysis, the factors independently associated with adverse relapse-free survival (RFS) were: bone marrow (BM)-WT1 ≥ 121/10(4) ABL copies (P = 0.02) and LAIP ≥ 0.2% (P = 0.0001) (after 1st consolidation) (RFS at the median follow up of 12.5 months: 51% vs. 82% [P
- Subjects :
- Oncology
Cancer Research
medicine.medical_specialty
medicine.diagnostic_test
business.industry
Myeloid leukemia
Retrospective cohort study
Bioinformatics
Minimal residual disease
Flow cytometry
03 medical and health sciences
0302 clinical medicine
Immunophenotyping
medicine.anatomical_structure
Median follow-up
hemic and lymphatic diseases
030220 oncology & carcinogenesis
Internal medicine
medicine
Radiology, Nuclear Medicine and imaging
Bone marrow
business
Survival analysis
030215 immunology
Subjects
Details
- ISSN :
- 20457634
- Volume :
- 5
- Database :
- OpenAIRE
- Journal :
- Cancer Medicine
- Accession number :
- edsair.doi...........051f27ab9ae880ea572f44d5edbf040b