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Nuclear proteins from a somatic and a germinal tissue of the echinoderm

Nuclear proteins from a somatic and a germinal tissue of the echinoderm

Authors :
Juan A. Subirana
Source :
Experimental Cell Research. 63:253-260
Publication Year :
1970
Publisher :
Elsevier BV, 1970.

Abstract

Histone fractions have been obtained from the haemal system and male gonads of the echinoderm Holothuria tubulosa . Fractionation was achieved by the methods of Johns [5], although some anomalies were found. The fractions were purified by precipitation in adequate solvent systems. The histones obtained are similar to those found in calf thymus, although f 1, and to a lesser extent f 2 b , show significant differences. In the gonads an additional minor component with high electrophoretic mobility is found. The other histone fractions appear to be identical with those obtained from somatic tissues. The relevance of this result towards an understanding of the role played by nuclear proteins in the sperm is discussed. Some of the acidic proteins present in the nuclei of these tissues are also preliminarily characterized.

Details

ISSN :
00144827
Volume :
63
Database :
OpenAIRE
Journal :
Experimental Cell Research
Accession number :
edsair.doi...........08c0c4d4c76d561b1b020e3b7911ab16
Full Text :
https://doi.org/10.1016/0014-4827(70)90211-9