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Effects of surface modification of upconversion nanoparticles on cellular uptake and cytotoxicity
- Source :
- Chemical Research in Chinese Universities. 32:474-479
- Publication Year :
- 2016
- Publisher :
- Springer Science and Business Media LLC, 2016.
-
Abstract
- Lanthanide-doped upconversion nanoparticles(UCNPs) are great promising to apply to biomedical imaging and therapy. We prepared NaYF4:Yb3+,Er3+ nanoparticles with different surface ligands, i.e., without any ligands(bare), coordinated with 2-aminoethyl dihydrogen phosphate(AEP), polyacrylic acid(PAA) or polyallylamine (PAAm), via a simple two-step ligand exchange of oleic acid capped NaYF4:Yb3+,Er3+ nanoparticles. Although the surface modification retained the crystal structure and transimission electron microscope(TEM) size distribution of the nanoparticles, and good dispersibility in aqueous solution and did not significantly change the upconversion luminescence, distinct differences were observed in the surface charge and hydrodynamic diameter. The cellular uptake and cytotoxicity of the nanoparticles were studied on two different cell lines, breast cancer MCF-7 and fibroblast 3T3. Confocal microscopy images demonstrate that PAAm-coordinated UCNPs can enhance the cellular uptake and endocytosis, whereas AEP- and PAA-coordinated UCNPs show a very low level of nonspecific adsorption. Biocompatibility studies based on 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay, however, indicate that PAAm-coordinated UCNPs are more toxic than the other two, and thus need further modifiaction(like PEG coordinating) to improve their biocompatibility. These results are important to the knowledge base required for the biomedical application of the UCNPs.
- Subjects :
- Aqueous solution
Biocompatibility
Ligand
Polyacrylic acid
Nanoparticle
Nanotechnology
02 engineering and technology
General Chemistry
010402 general chemistry
021001 nanoscience & nanotechnology
01 natural sciences
Photon upconversion
0104 chemical sciences
chemistry.chemical_compound
chemistry
Biophysics
Surface modification
Surface charge
0210 nano-technology
Subjects
Details
- ISSN :
- 22103171 and 10059040
- Volume :
- 32
- Database :
- OpenAIRE
- Journal :
- Chemical Research in Chinese Universities
- Accession number :
- edsair.doi...........09c0aa20ded2e2964ad9205c5440f2e3