Additional file 1: of Production and purification of chimeric HBc virus-like particles carrying influenza virus LAH domain as vaccine candidates

Determination of copy number for integrated expression units in selected P. pastoris clones. Description of the methodology used for quantification of copy number for expression cassettes integrated in selected P. pastoris clones. The method is based on real time PCR amplification of zeocin gene. (DOCX 186 kb)