Molecular Methods in Myeloproliferative Neoplasms

In this chapter molecular methods that are clinically useful in the diagnosis of myeloproliferative neoplasms are presented. The methods described are the essential elements of contemporary hematological diagnostic procedures and allow a diagnosis to be reached more easily and precisely. Detailed protocols for the detection of mutations in JAK2, MPL, and KIT genes, as well as for the detection of the fusion gene FIP1L1/PDGFRA, are presented.