Abstract 763: Cranberry juice constituents as an adjuvant for protein vaccination against malignant lymphoma

Cranberry fruit and juice have been reported to exert a multitude of health-related benefits. NDM is a 12-30K molecular weight fraction, which we have derived from cranberry juice and which, as we have previously reported, impairs in vitro growth and invasion through extracellular matrix of murine lymphoma cells [N.Hochman et al. Nutrition and Cancer 60(4), 511-517,2008]. Furthermore, intraperitoneal inoculation of NDM inhibited the in vivo growth of these tumors in syngeneic hosts, demonstrating its in vivo efficacy as an anti-tumorigenic agent. MMTV-p14 (p14 for short) is the signal peptide of the env-precursor of Mouse Mammary Tumor Virus. This peptide was found by us to localize in the nucleoli of every MMTV-containing lymphoma or mammary carcinoma tested, (as well as in some cases of human breast cancer). Here we report that priming and boosting of mice with purified recombinant p14 +NDM results in enhanced generation of anti-p14 antibodies in the sera of inoculated mice. Furthermore, mice primed and boosted with p14+NDM are immunized against a subsequent challenge with malignant lymphoma cells that contain MMTV. Mice, primed and boosted separately with p14 or NDM were not immunized against a lymphoma challenge. These findings demonstrate that NDM can be used as adjuvant in a protein vaccination protocol against malignant lymphoma cells that harbor the virus MMTV. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 763. doi:10.1158/1538-7445.AM2011-763