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Technical note: Comparison of automated ribosomal intergenic spacer analysis and denaturing gradient gel electrophoresis to assess bacterial diversity in the rumen of sheep1

Technical note: Comparison of automated ribosomal intergenic spacer analysis and denaturing gradient gel electrophoresis to assess bacterial diversity in the rumen of sheep1

Authors :
M. J. Ranilla
Cristina Saro
Ana Cifuentes
Ramon Rosselló-Móra
María Dolores Carro
Source :
Journal of Animal Science. 92:1083-1088
Publication Year :
2014
Publisher :
Oxford University Press (OUP), 2014.

Abstract

The aim of this study was to compare automated ribosomal intergenic spacer analysis (ARISA) and denaturing gradient gel electrophoresis (DGGE) techniques to assess bacterial diversity in the rumen of sheep. Sheep were fed 2 diets with 70% of either alfalfa hay or grass hay, and the solid (SOL) and liquid (LIQ) phases of the rumen were sampled immediately before feeding (0 h) and at 4 and 8 h postfeeding. Both techniques detected similar differences between forages, with alfalfa hay promoting greater (P 0.05) over the postfeeding period were detected by DGGE. The ARISA technique showed lower (P 0.05) in bacterial diversity between both rumen phases were detected by DGGE. Under the conditions of this study, the DGGE was not sensitive enough to detect some changes in ruminal bacterial communities, and therefore ARISA was considered more accurate for assessing bacterial diversity of ruminal samples. The results highlight the influence of the fingerprinting technique used to draw conclusions on factors affecting ruminal bacterial diversity.

Details

ISSN :
15253163 and 00218812
Volume :
92
Database :
OpenAIRE
Journal :
Journal of Animal Science
Accession number :
edsair.doi...........0d03dc7c86a0c2e9586c0f4432bb28d6
Full Text :
https://doi.org/10.2527/jas.2013-7175