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Technical note: Comparison of automated ribosomal intergenic spacer analysis and denaturing gradient gel electrophoresis to assess bacterial diversity in the rumen of sheep1
Technical note: Comparison of automated ribosomal intergenic spacer analysis and denaturing gradient gel electrophoresis to assess bacterial diversity in the rumen of sheep1
- Source :
- Journal of Animal Science. 92:1083-1088
- Publication Year :
- 2014
- Publisher :
- Oxford University Press (OUP), 2014.
-
Abstract
- The aim of this study was to compare automated ribosomal intergenic spacer analysis (ARISA) and denaturing gradient gel electrophoresis (DGGE) techniques to assess bacterial diversity in the rumen of sheep. Sheep were fed 2 diets with 70% of either alfalfa hay or grass hay, and the solid (SOL) and liquid (LIQ) phases of the rumen were sampled immediately before feeding (0 h) and at 4 and 8 h postfeeding. Both techniques detected similar differences between forages, with alfalfa hay promoting greater (P 0.05) over the postfeeding period were detected by DGGE. The ARISA technique showed lower (P 0.05) in bacterial diversity between both rumen phases were detected by DGGE. Under the conditions of this study, the DGGE was not sensitive enough to detect some changes in ruminal bacterial communities, and therefore ARISA was considered more accurate for assessing bacterial diversity of ruminal samples. The results highlight the influence of the fingerprinting technique used to draw conclusions on factors affecting ruminal bacterial diversity.
- Subjects :
- 2. Zero hunger
0303 health sciences
030306 microbiology
Ribosomal Intergenic Spacer analysis
food and beverages
Forage
Technical note
General Medicine
Biology
Microbiology
03 medical and health sciences
Rumen
Alfalfa hay
Genetics
Hay
Animal Science and Zoology
Food science
Temperature gradient gel electrophoresis
030304 developmental biology
Food Science
Subjects
Details
- ISSN :
- 15253163 and 00218812
- Volume :
- 92
- Database :
- OpenAIRE
- Journal :
- Journal of Animal Science
- Accession number :
- edsair.doi...........0d03dc7c86a0c2e9586c0f4432bb28d6
- Full Text :
- https://doi.org/10.2527/jas.2013-7175